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In response to increased white-tailed deer (Odocoileus virginianus) encroachment on airports, we evaluated the effectiveness of cattle guards as deer exclusion devices. We conducted three experiments in a 2200 ha fenced facility in northern Ohio with high (91/km2) deer densities during 1994 ± 1995. In each experiment, we monitored deer crossings at two or three cattle guards (4.6[L] x 3[W]x 0.5 or 1.0[D] m) constructed at fence openings for 2 weeks pre- and post-installation. For each experiment, the mean daily number of deer crossings after installation of cattle guards was reduced (P < 0.01) by > 88% compared with respective crossing rates during pretreatment. Reduction in deer crossings using cattle guards with 0.5 or 1.0 m deep excavations were similar (95 ± 96% vs 98%) overall. Cattle guards at permanent openings used for vehicular traffic appear a viable technique to reduce deer movements into fenced airports and other facilities where reductions in deer intrusions are desired.