U.S. Department of Agriculture: Animal and Plant Health Inspection Service


Date of this Version

August 2005


Methods are developed to extract and quantitate the avicide 3-chloro-p-toluidine hydrochloride (CPT HCI) from rough-hulled rice and ethyl-cellulose-coated rice baits using high-performance liquid chromatography. The mobile phase used in the ethyl-cellulose-coated rice matrix method is an acetonitrile(ACN)-phosphate buffer (60:40) at pH 8, and the rough-hulled rice matrix method uses an ACN-phosphate (70:30) buffer at pH 2. Increased retention time is observed for CPT HCI at the higher pH. The two methods have been useful in characterizing different bait formulations in an ongoing pesticide formulation improvement program.