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Today, e-journals play a significant role in meeting information needs of scholars. On the other hand, each year publishers demand higher costs for e-journals subscription. Librarians seek ways to assess the use of e-journals in order to determine how well they meet users' needs and to justify increases in budget for e-journals acquisition. This study aimed to assess accessibility and use of e-journals by the research scholars at top ten university libraries affiliated to the Ministry of Science, Research and Technology (MSRT) in Iran. Questionnaire was adopted for data collection and a total of 1380 research scholars participated in this study. It was found that the level of e-journals accessibility at university libraries affiliated to the MSRT in Iran was high. A great majority of research scholars were aware of e-journals at their libraries. Further, e-journals had been used more than medium level. Although research scholars belonging to Basic Sciences were known as the heaviest e-journals users, available e-journals met their information needs less than the other disciplines. The study suggested that the provision of e-journals have to be maintained and continued by Iranian university libraries concerning users’ preferences and characteristics especially users’ discipline.