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This study investigates rural women’s use of indigenous knowledge in Nigeria, with particular reference to the Isoko ethnic nationality of Delta State. The survey research design was adopted for the study. A questionnaire was designed to collect data from women located in ten rural communities of Isoko South Local Government Area of Delta State that constitute the sample size for this study. Analyses were carried out using frequencies and percentage distributions. Findings reveal an extensive wealth and use of indigenous knowledge in agriculture, health care, child delivery, economic and child care. The study also reveals that majority of rural women dwellers are non-literate because they lack minimum basic education. The findings provide a platform for the development and application of indigenous knowledge for sustainable development in Nigeria