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A well-managed organization sees employees as the source of quality and productivity. This study examines the perception of work motivation in relation to job satisfaction and organizational commitment of library personnel in academic and research libraries in Oyo state, Nigeria. Two hundred library personnel (41% female, 59% male) were selected through a total enumeration sampling from five research and four academic libraries. A questionnaire called Work Motivation, Job Satisfaction, and Organizational Commitment Scale (WMJSCS) with the overall co-efficient of r = 0.83 cronbach alpha was adapted from Organization Commitment Questionnaire by Mooday et al. (1979), Minnesota Satisfaction Questionnaire by Weiss, 1967, and Motivation Behaviour Scale of Akinboye, 2001. The Executive Behaviour Battery was used for data gathering. Four research questions were developed and analyzed using multiple correlation/classification, multiple regression, and t-test statistical tools. The result indicates that both job satisfaction and commitment correlate with perception of work motivation by library workers. No significant difference exists in the perception of work motivation of professional and non-professional library personnel. Employees in academic libraries and research libraries have the same level of job satisfaction. Years of experience have no relationship with commitment.