The present paper investigates Bradford’s Law of scattering for the ‘Microbiology’ literature in India for the period 2002-2016 as accessible in the Web of Science Core Collection database. A total of 25,744 articles related to Microbiology literature published in journals during the study period is retrieved. The data has been examined with respect to year-wise publication productivity, Relative Growth Rate (RGR) and Doubling Time (DT) of literature. The time series analysis is also used to predict the future growth of literature. The 25,744 articles are scattered in 328 journals. A top ranked journals list was prepared and it unveiled that the Bio-resource Technology Journal has emerged as the most preferred journal with 1,610 (6.25%) articles followed by Applied Biochemistry and Biotechnology with 1,019 (3.95%) articles and World journal of Microbiology Biotechnology with 746 (2.89%) articles. Theoretical aspects of Bradford’s Law of Scattering are verified and manifested that the data does not fit to the present sample. The Leimkuhler model is applied and it proved to the data set for the Bradford Multiplier (k) at 14.17. The Bradford law is also verified through graphical formulation by drawing the graph and is found to approve all the three characteristics.