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Scientometric is a type of research method used in the library and information science. The study of the use of documents and patterns of publication in which mathematical and statistical methods have been applied in the scientometric study. It is based on quantitative analysis and statistics to describe patterns of publication within a given field. The study presents the trends of authorship pattern and collaborative research activity in Biotechnology in ISBA Countries. A sample of 24888 articles collect from Scopus database during 2007-2016 is analyzed. The applying scientometric tools are; Collaboration Coefficient, Authorship pattern and Activity Index. In the ten years period, the multi-authorship articles are higher and dominant than single authorship pattern. The average Activity Index of IBSA countries for ten years, South Africa computed highest, where the India and Brazil took second and third place. The international collaboration also presented in this article and it is shown that for IBSA countries USA is most collaborated country.