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This study evaluates the Applied and Environmental Microbiology (AEM). Applied and Environmental Microbiology is a biweekly peer-reviewed scientific journal published by the American Society for Microbiology. ASM's mission is to promote and advance the microbial sciences. The present bibliometrics study was 4985 scholarly research articles published in ‘Applied and Environmental Microbiology Journal, during the period 2001-2005. The collected data were analyzed with the help of ‘Bibexcel tool’ and Hist cite. The references were collected from Mendeley and Endnote Software. The study also applied statistical tools such as Authorship pattern, Relative Growth Rate, Collaborative Index, Degree of Collaboration, Zipf Law, and Exponential Growth Rate.