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Highly challenging and unpredictable conditions in clinical setting impose excessive psychological pressure on employees who are working in these sectors. Such conditions can lead to serious consequences such as occupational burnout. This study aimed to determine the level of occupational burnout in clinical nurses and emergency technicians. This cross-sectional study was conducted on 154 clinical nurses and 114 emergency technicians in 2015. In this study we used two data collection tools including Maslach burnout Inventory and a demographic questionnaire. Data were analyzed using Pearson correlation coefficient and independent t-test.The majority of employees in both groups had a moderate level of occupational burnout (in all aspects). Emergency technicians had higher levels of occupational burnout than nurses however except for emotional exhaustion there was no statistically significant difference between the two groups in terms of other dimensions and the total score. Moreover, occupational burnout in both groups had a significant positive correlation with age and work experience. Work hours had a significant relationship with occupational burnout in nurses, but it had no significant relationship with occupational burnout in emergency technicians. Concerning occupational burnout, there was only a significant difference between the two groups in terms of emotional exhaustion. However given the prevalence of this syndrome in both groups, it is necessary to identify and modify the influencing factors so that to control the syndrome and achieve a better level of work quality.