This paper examines the role of libraries in the preservation and accessibility of the indigenous knowledge in the Niger Delta region of Nigeria. The survey method was used for the study and data were collected with the questionnaire from 82 respondents drawn from six libraries in the region. Niger Delta people use indigenous knowledge to improve agricultural production, acquire education and basic skills, conserve their natural environment, and resolve conflicts. Libraries in the Niger Delta region do not manage indigenous knowledge resources effectively. To preserve and make indigenous knowledge accessible, libraries should collaborate with indigenous people to record indigenous knowledge, store it, and make it available through television and radio broadcast, the Internet, exhibits, or mobile library services.