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For human resources to function effectively and efficiently, they need to have the requisite skills and abilities which could come by way of capacity building. Hence, this study examined Tertiary Education Trust Fund (TETFund) interventions and capacity building for librarians in public university libraries in South-East, Nigeria. The study adopted a descriptive survey research design. The population of the study was 180 respondents selected randomly from the ten public university libraries in South East, Nigeriaand complete enumeration was adopted. The instrument for data collection was a self-designed structured questionnaire which was validated using face and content validity and the reliability was ascertained using Crombach Alpha. The data was analysed with a combination of descriptive and inferential statistics. The hypotheses were tested using parametric Chi-square at 5% level of significance. The findings showed that TETFund intervention play a significant role in capacity building of librarians and that capacity building has significantly assisted librarians in discharging their duties in public universities in South-East, Nigeria. Sequel to this, it was recommended among other things that the number of librarians that gain from capacity building intervention of TETFund needs to be increased through increased funding by the agency.