Objective: Promoting health and determining the level of health literacy is one of the most important indicators in each society. This study was conducted with the aim of evaluating the health literacy status among librarians of Azad universities and medical sciences regarding the impact of librarians on their individual health and their role in disseminating health information that promotes health. This research is a descriptive survey. The statistical population consists of 90 librarians working in the libraries of the Islamic Azad universities and medical sciences in Tehran. The data were collected using TOFHLA's functional health literacy questionnaire in December 2018 Descriptive statistics, one-way T-test and Friedman test were used to analyze the data. The mean age of the subjects was 39, of which 74.7% were women and the rest were male, and 69% had a library education. The mean health literacy score was 83.7%. 82.3% of librarians had adequate health literacy, 3.8% had border health literacy and 13.9% had inadequate health literacy. The health literacy of librarians in two universities was sufficient and not significant. Conclusion: Since there was no significant difference between the health literacy of librarians of the two universities, the activity in medical science libraries was not very effective in achieving health literacy.