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Electronic resources are transforming most academic library systems and how students view and use these information sources is critical. The study thus explored students’ perceptions and practices regarding the use of electronic resources in public university libraries in Ghana. The study adopted the descriptive research design, and the quantitative research approach was used; hence questionnaires were the main instrument employed for data collection. A total of 537 students were conveniently sampled from six public universities. From the results, it was found that most of the public universities have Web OPAC systems, e-books, e-databases, and e-journals available for students’ use and that most of the public libraries are constrained by insufficient computer systems, difficulties in detecting the relevant electronic resources to meet students information needs, lack of skills in using the electronic resources, and unreliable internet facilities/connectivity (access). Confirming the challenge of students regarding the use of electronic resources, the study observed that the availability of electronic resources as well as ease of use negatively influenced student’s willingness to patronize electronic resources. By implication, university authorities must ensure libraries are adequately resourced to facilitate the effective operation of electronic resource systems in the universities. Staff and students alike also need to be continually trained on the various electronic resource technologies deployed for use. The institution, as well as librarians, must endeavour to create awareness regarding the relevance of the electronic resources to academic work in the institutions.