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This study investigated the influence of ownership structure and leadership styles on turnover intention of Library and Information Science (LIS) Professionals in private universities in Osun State, Nigeria. Descriptive and inferential statistics of frequency counts, percentages and correlational methods were employed to analyse the data gathered from 59 LIS professionals in the State. The indices of ownership structure (functional, divisional, matrix and hybrid) and leadership styles (democratic, autocratic, transactional) were found to have positive linear correlations with turnover intention of LIS professionals. Deployment of varying leadership styles, promotion of ‘entity concept’ and facilitation of inter-relationship among professionals were suggested.