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This study investigated the effect of e-literacy maturity level (Information Literacy, Media Literacy, Moral Literacy, and Learning & Thinking Skills) on the lecturers’ information use behavior at UIN Syarif Hidayatullah Jakarta, Indonesia. The research based on Social Cognitive Theory (SCT) and Uses and Gratification Theory (UGT) had been exploring the relations and effects of the e-literacy maturity level to the information use behavior as a communication process in electronic media. A questioner survey based on a cluster sample of 91 lecturers at 11 faculties had been taken for this explanatory research. The result showed that the mean score for all of the constructs was 4 (high) out of maximum score obtainable of 5 (very high). Simultaneously, the effects of e-literacy maturity level, as a characteristic factor in SCT and as an antecedent in UGT, to the information use behavior are significant, f-value (39,04) > f-table (2,70). Partially, the effect of Information Literacy and Learning & Thinking Skill on the information use behavior are significant, t-value (2,486; 4,237) > t-table (1,985), but the Media Literacy and Moral Literacy are not. Meanwhile, there are significant relationships between all sub-variable in e-Literacy affecting Information Use Behavior. Therefore, it’s recommended that the entire sub-variables of e-literacy as one that is important in the formation of a motivation training for using of electronic media for the lecturers.