The purpose of this paper was to explore the current assessment practices of information literacy instruction (ILI) in the libraries of universities in Pakistan. The research method consisted of a quantitative approach using a structured questionnaire for empirical data collection, which was sent to the libraries of universities of Pakistan. The findings of the study show that university libraries evaluated their information literacy instruction programs to find out the gaps and deficiencies through student and teachers’ feedback. Student learning was also assessed through faculty feedback and oral feedback. The outcome of this study will help the Library and Information Sciences (LIS) schools, library associations, and universities comprehend the present status of information literacy instructions assessment in the libraries of Pakistani universities.