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Manuscripts are the major source of information to know about the historical facts, cultural and old traditions of the past. They are scattered all over the world and in India about six million manuscripts are present in different languages and scripts. Therefore, steps have been taken to conserve and preserve the available manuscripts by every possible technique in the libraries. The present study examined the process of conservation and preservation of manuscripts adopted at National Library of India (NL) and Rampur Raza Library (RRL) along with the present conditions of manuscripts using the questionnaire and personal visit as the sources of data collection. The study revealed that both the libraries are adopting different techniques for the conservation of manuscripts and in some way they are successful in protecting the manuscripts.