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The aim of this paper was to explore the factors affecting knowledge production, diffusion and utilisation in a university environment taking the University of Zambia Medical School as a case of study. Methodologically, a survey of lecturers was carried out. Data was collected using a semi structured questionnaire; and analysed using MS Excel which was later presented in simple statistics of figures and graphs. The study established that knowledge production, diffusion and utilisation was affected by inadequate funding, time, interest, technology, availability of appropriate reading materials, incentives, internet research skills, heavy workload and lack of publication outlets. The study contributes to the understanding of the context of the factors that may play a negatively role in the knowledge production, diffusion and utilisation practices in Universities.