The study aimed to evaluate the bibliometric research of Library and Information Science (LIS) research contributed by the authors affiliated with the University of Karachi (UoK), Pakistan. The dataset was limited to 22 years from January 2000 to December 2021 and a list of evaluated papers has been proved from various online and print sources. Google Scholar has been employed to access the record of citation(s). Ninety-two papers were identified with at least one author affiliated with the UoK on the subject category of LIS and these papers were cited 707 times. Two-author collaboration was found to be the preferred authorship pattern and the papers written in this pattern got a better citation result. A number of research articles' contributions have been found that female authors are more than male authors. Syed Jalaluddin Haider, Munira Nasreen Ansari and Farhat Hussain emerged as the most prolific authors with 26, 25 and 15 papers, respectively. The papers published in international journals got higher citations and about three-fourth of the papers were published in the top 11 sources which in found from these research. The subject dispersion revealed that LIS Education and Library Management were the favorite areas. The characteristics of the ten most cited papers revealed that 60% citations were gained by these papers. The findings of this paper support understanding the research trends in LIS at UoK. There is a need to accelerate the research activities, revisit the research policies and promote the research culture in the UoK.