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The study investigated gender differences in information literacy self-efficacy among academic librarians working in federal university libraries in South-east Nigeria. The descriptive survey research design was used in this study. Using purposive sampling, twenty academic librarians were selected for the study. The Information Literacy Self-Efficacy Scale (ILSES) was used to obtain the data. In total, there are 28 items on the scale, which has a high Cronbach Alpha reliability score of 0.91. In this scale, seven sub-dimensions are measured extensively. To analyze the data, percentages, means, standard deviations, t-tests, and Lavene's tests were used. In terms of mean ratings, male and female academic librarians did not differ significantly (2,28) = -183; p =.855. As such, gender has no effect on information literacy self-efficacy in academic librarians. Any difference in the perceived self-efficacy of academic librarians in information literacy was not caused by their gender. To improve work efficiency and professional competency, academic librarians should upgrade their information literacy skills.