Mechanical & Materials Engineering, Department of
Date of this Version
7-7-2022
Citation
Methods Mol Biol. 2020 ; 2050: 91–97. doi:10.1007/978-1-4939-9740-4_10.
Abstract
Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells yet results in variable levels of viability and transfection efficiency across the population. Localized electroporation is an alternative that can be administered on a similar scale but results in much more consistent with higher quality transfection and higher cell viability. This paper discusses the creation and use of a simple and cost-effective device using porous membrane for performing localized electroporation.
Included in
Mechanics of Materials Commons, Nanoscience and Nanotechnology Commons, Other Engineering Science and Materials Commons, Other Mechanical Engineering Commons
Comments
PMCID: PMC9262042