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Absorbance and reflectance spectra in the visible and near infrared range of the spectrum, acquired for maple (Acer platanoides L.) leaves were studied. Standard deviation of absorbance spectra showed that in yellow to green leaves, with chlorophyll content at least up to 30 nmol/cm2, there is a spectral feature at 520 nm attributable to carotenoids. Reflectance around 520 nm also correlates closely with carotenoids content in yellow to green leaves. Thus, this spectral feature at 520 nm could be used as a measure of carotenoids content in green leaves and plants.