Fate of antimicrobials and antimicrobial resistance genes in simulated swine manure storage

Authors

Stacey R. Joy, University of Nebraska-Lincoln
Xu Li, University of Nebraska-LincolnFollow
Daniel D. Snow, University of Nebraska-LincolnFollow
John Gilley, USDA-ARSFollow
Bryan L. Woodbury, USDA‐ARS, USMARC, Clay Center, NEFollow
Shannon L. Bartelt-Hunt, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2014

Citation

Science of the Total Environment 481 (2014) 69–74; doi: 10.1016/j.scitotenv.2014.02.027

Comments

This article is a U.S. government work, and is not subject to copyright in the United States.

Abstract

The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from1 day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% of the starting concentration by day 40; however, the relative abundance of erm(B) remained at 50–60% of the initial relative abundance while the relative abundance of erm(F) decreased by 80–90%, consistent with tylosin. These results indicate that tet resistance genes respond primarily to chlortetracycline antimicrobials, and may be lost when the parent tetracycline compound is degraded. In contrast, erm(B) resistance gene may respond to a range of antimicrobials in animal manure, and may persist despite losses of tylosin.