Natural Resources, School of


Date of this Version

February 2003


Citation: Iñiguez AM, Reinhard KJ, Araújo A, Ferreira LF, Vicente ACP (2003) Enterobius vermicularis ancient DNA from North and South American human coprolites. Memorias do Instituto do Oswaldo Cruz 98:67-69.


A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.