Agronomy and Horticulture Department

 

Title

Application of Molecular Marker Technology to QA/QC in Plant Breeding

Authors

Date of this Version

2019

Document Type

Article

Citation

Plant and Soil Sciences eLibrary (PASSeL) Lessons.

Comments

Copyright 2019, Plant and Soil Sciences eLibrary. Used by permission.

This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, administered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or NSF.

This eLesson was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, administered by the University of California-Davis. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA -NIFA.

Abstract

Introduction

Quality Assurance and Quality Control (QA QC) are key for developing effective plant breeding programs which make the most impact for the resources available. Molecular markers can greatly enhance Quality Assurance and Quality Control in plant breeding. It is therefore important to know what is meant by QA QC, how markers are used in QA and QC, how to assess the resultant data, and how to make decisions on selecting lines to move forward in the breeding-to-market process.

This lesson is written for plant breeders who are interested in or beginning to use molecular marker technology in quality assurance and quality control in plant breeding.

Objectives:

At the end of this lesson you will be able to:

  • Define what is meant by Quality Assurance and Quality Control
  • Describe how molecular markers can assist in Quality Assurance and Quality Control
  • Explain how to effectively utilize markers including how many to use and at which stage in the breeding processes they can be used
  • Assess the results of genotyping data and make decisions from that data

Summary

While quality assurance (QA) and quality control (QC) have similar goals they are both focused on different aspects of a plant breeding process. In this lesson we covered the role and application of markers in improving QA and QC for a plant breeding program as well as the verification processes required to establish the true identity and genotypes of various germplasm created e.g parental lines, breeding populations, yield lines, etc,. It is essential that breeding data generated by markers is understood and interpreted correctly so as to assure the QC process-thus several examples of tabular data were used in this lesson with explanations that guides the reader in understanding SNP marker generated breeding data before seed production and marketing can start.

Modules:

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