Agronomy and Horticulture Department



Transformation 2 - Transformation Methods

Date of this Version


Document Type



Plant and Soil Sciences eLibrary (PASSeL) Lesson


Copyright © 2003 Patricia Hain and Don Lee. Used by permission.

Peer reviewed web lesson JNRLSE approved 2003

Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept. of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture.


Overview and Objectives – Transformation

This lesson explains the procedure of introducing a new gene into a plant cell (transformation). It discusses the main goals of the transformation process and describes the four main methods of transformation.

At the completion of this lesson you should be able to:

  • Describe what must happen for a cell to be successfully transformed. (transformation goals)
  • List and explain four of the current methods used in transformation.
  • Explain why Agrobacterium is considered a “natural genetic engineer.”