Gene Cloning Part 1: The Mechanics of Recombinant DNA
Date of this Version
Plant and Soil Sciences eLibrary (PASSeL) Lesson
Objectives for Gene Cloning Part 1
This lesson describes how DNA molecules can be recombined to make recombinant DNA and how special DNA molecules called plasmids allow scientists to clone genes.
Objectives: At the completion of this lesson you should be able to:
- Define gene cloning, gene library, plasmid, recombinant plasmid, and vector.
- Outline and describe the steps of the process used to make genomic and cDNA libraries.
- Explain the role of antibiotic resistance and selectable marker genes in the gene cloning process.
- Contrast libraries that use plasmids as vectors to BAC or YAC libraries.
- Lesson home
- Objectives for Gene Cloning Part 1
- The Gene Cloning Process
- Why clone genes?
- DNA is cloned from DNA
- Genes are Connected to Other Genes
- Gene Cloning Requires Knowledge of the Gene’s Unique Characteristics.
- Plasmids do the “Tricking”
- Cloning with LacZ and X-gal plasmids
- Step One: Make Sticky Ends
- Step Two: Make Recombinant Plasmids
- Step Three: Transformation of E. coli
- Step Four: Selecting Bacteria with Recombinant Plasmids
- Step Five: Making Libraries of Genes
- Genomic Libraries
- cDNA library
- Gene Libraries with Big Pieces