Agronomy and Horticulture Department



Gene Design 2 - Gene Constructs

Date of this Version


Document Type



Plant and Soil Sciences eLibrary (PASSeL) Lesson


Copyright © 2003 Patricia Hain and Don Lee. Used by permission.

Peer reviewed web lesson JNRLSE approved 2003

This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, administered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA or NSF.

Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept. of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.


Overview and Objectives - Gene Constructs

This lesson builds upon the gene region lesson discussing the gene construct of currently used hybrids and explaining how these combinations result in a particular gene expression.

At the completion of this lesson, you should be able to:

  • List four considerations genetic engineers must keep in mind when designing genes and which gene region controls each.
  • Explain the difference between input and output traits and give an example of each.
  • Predict how modification of a gene will influence gene expression, specifically which protein is produced, when it is produced, and how much is produced.
  • Identify the gene construct and explain the expression of Bt, Liberty resistant, and Roundup-resistant lines.
  • Describe how transgene expression can cause undesired effects (yield drag).