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We have observed an enhancement of the temperature effect on magnetoresistance (MR) in Fe/Mo multilayers with the increase of the Mo layer thickness. MR at the first antiferromagnetic maximum is large and insensitive to the temperature whereas at the third antiferromagnetic maximum it is small and relatively sensitive to the temperature. We found that the mean free path of the conduction electrons increased with the increase of the Mo layer thickness at a fixed temperature and it decreased very slightly with the increase of the temperature at a fixed Mo layer thickness. The interlayer coupling, similar to MR, also has an enhanced temperature effect with the increase of the Mo layer thickness. There is a scaling relationship between the temperature effect on saturation field Hs and MR, which suggests that the decrease of the interlayer coupling strength is responsible for the enhanced temperature effect on MR.