Reclassification of Subspecies of Acidovorax avenae as A. Avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911)comb.nov., A. citrulli Schaad et al.,1978)comb.nov., and proposal of A. oryzae sp. nov.

Authors

Norman Schaad, USDA-ARS
Elena Postnikova, USDA-ARS
Aaron Sechler, USDA-ARS
Larry E. Claflin, Kansas State University
Anne K. Vidaver, University of Nebraska-LincolnFollow
Jeffrey B. Jones, UniversityofFlorida,USA
Irina V. Agarkova, University of Nebraska-LincolnFollow
Alexander Ignatov, Center ‘‘Bioengineering’’ RAS, Moscow, Russia
Ellen Dickstein, University of Florida
Bruce A. Ramundo, Kansas State University

Date of this Version

2008

Comments

Published in Systematic and Applied Microbiology 31 (2008) 434–446.

Abstract

The bacterium Acidovorax avenae causes disease in a wide range of economically important monocotyledonous and dicotyledonous plants, including corn, rice, watermelon, anthurium, and orchids.Genotypic and phenotypic relatedness among strains of phytopathogenic A. avenae sub sp. avenae, A. avenae sub sp. citrulli, A. avenae subsp. cattleyae and A. konjaci, as well as all other Acidovorax species, including A. facilis, the type strain of Acidovorax, was determined.The16s rDNA sequencing confirmed previous studies showing the environmental species to be very distant from the phytopathogenic species. DNA/DNA reassociation assays on the different strains of A. avenae revealed four(A, B, C, and D) distinct genotypes. Taxon A included six A. avenae subsp. avenaestrains from corn that had a mean reciprocal similarity of 81%; taxon B included six A. avenae sub sp. avenae strains from rice that had a mean reciprocal similarity of 97%; taxon C contained 11 A. avenae sub sp. citrulli strains from cucurbits (cantaloupe, watermelon, and pumpkin) that had a mean reciprocal similarity of 88%, and taxon D contained four A. avenae sub sp. cattleyae strains from orchids that had a mean similarity of 98%.