Plant Pathology Department
Date of this Version
2002
Abstract
The isolation and characterization of a virus (designated EhV) that infects the marine coccolithophorid Emiliania huxleyi (Lohmann) Hay & Mohler are described. Three independent clones of EhV were isolated from Norwegian coastal waters in years 1999 and 2000. EhV is a double-stranded DNA-containing virus with a genome size of ~415 kilo-base pairs. The viral particle is an icosahedron with a diameter of 160–180 nm. The virus particle contains at least nine proteins ranging from 10 to 140 kDa; the major capsid protein weighs ~54 kDa. EhV has a latent period of 12–14 h and a burst size of 400–1000 (mean, 620) viral particles per cell. A phylogenetic tree based on DNA polymerase amino acid sequences indicates EhV should be as¬signed to the Phycodnaviridae virus family and that the virus is most closely related to viruses that infect Micromonas pusilla and certain Chlorella species.
Comments
Published in Journal of Phycology 38:4 (2002), pp. 767–774; doi 10.1046/j.1529-8817.2002.02015.x Copyright © 2002 Phycological Society of America, Inc.; published by John WIley & Sons. Used by permission. http://www3.interscience.wiley.com/journal/118502413/home