Characterization of the large (L) RNA of peanut bud necrosis tospovirus

Authors

S. Gowda, University of Florida
Satyanarayana Tatineni, USDA-ARSFollow
R. A. Naidu, lInternational Crops Research Institute for the Semi-Arid Tropics
A. Mushegian, National Institute of Health
W. O. Dawson, University of Florida
D. V. R. Reddy, International Crops Research Institute for the Semi-Arid Tropics

Date of this Version

1998

Citation

Arch Virol (1998) 143: 2381–2390.

Comments

U.S. government work.

Abstract

The nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (PBNV), a member of the genus Tospovirus, contained both viral(v) and viral complementary(vc) sense L RNAs. Defective forms of L RNA containing ‘core polymerase region’ were observed. The full length L RNA of PBNV was sequenced using overlapping cDNA clones. The 8911 nucleotide L RNA contains a single open reading frame (ORF) in the vc strand, and encodes a protein of 330 kDa. At the 50 and 30 termini of the v sense RNA there were 247 and 32 nt untranslated regions, respectively, containing an 18 nt complementary sequence with one mismatch. Comparisons of the predicted amino acid sequence of the L protein of PBNV with other members of Bunyaviridae suggest that the L protein of PBNV is a viral polymerase. The L protein had highest identity in the ‘core-polymerase domain’ with the corresponding regions of other tospoviruses, tomato spotted wilt virus and impatiens necrotic spot virus.