Semiconservative Synthesis of Single-Stranded RNA by Bacteriophage φ6 RNA Polymerase

Authors

James L. Van Etten, University of Nebraska-LincolnFollow
Dwight E. Burbank, University of Nebraska-Lincoln
Diane A. Cuppels, University of Nebraska-Lincoln
Leslie C. Lane, University of Nebraska-LincolnFollow
Anne K. Vidaver, University of Nebraska-LincolnFollow

Date of this Version

2-1-1980

Comments

Published in JOURNAL OF VIROLOGY, Feb. 1980, p. 769-773 Vol. 33, No. 2. Copyright 1980. Used by permission.

Abstract

The RNA polymerase in the nucleocapsid of seudomonas phaseolicola bacteriophage φ6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small singlestranded RNAs were synthesized from each double-stranded RNA.