Date of this Version
Aedes triseriatus mosquitoes were trapped and processed for viral isolation. Swiss white mice and BHK-21 cell cultures were used for propagation of virus. A sucrose-acetone extracted antigen was produced from infected mouse brains. Anti-serum was produced in Swiss white mice as hyperimmune ascitic fluid using Sarcoma 180/TG. The isolate was identified using hemagglutination, hemagglutination-inhibition, and serum neutralization techniques. The virus was identified as California group arbovirus, type Trivittatus.
This is the first reported isolation of California group arbovirus, type Trivittatus virus from field samples of A. triseriatus mosquito and the first time the virus has been isolated from any species of mosquito in the state of Nebraska.