Determining Gene Specific Chromatin Differences in Sulfolobus solfataricus: Expression of MerR Protein for Targeted-ChIP Antibody Production

Authors

Erica M. North, University of Nebraska - LincolnFollow
Sophie Payne, University of Nebraska - LincolnFollow
Sam McCarthy, University of Nebraska - Lincoln
Tyler Johnson, University of Nebraska - LincolnFollow
Paul H. Blum, University of Nebraska - LincolnFollow

Date of this Version

5-2016

Document Type

Poster

Citation

UCARE Poster session, University of Nebraska-Lincoln Research Fair, April 2016, Lincoln, NE.

Comments

Copyright © 2016 Erica North, Sophie Payne, Sam McCarthy, Tyler Johnson, Paul Blum

Abstract

In this project the repressor protein MerR from the Sulfolobus solfataricus mercury resistance operon was cloned into pET28b and transformed into Roetta 2 E.coli strains for overexpression and purification. Large quantities of recombinant MerR will be used for subsequent injection into a mammalian host for antibody production. These antibodies will be used in targeted-ChIP studies in which gene specific chromatin modification states will be analyzed. The overproduction of MerR is part of a larger project where future research could produce data on whether gene expression levels and chromatin modifiation states could be correlated at an individual gene level, possibly suggesting a novel epigenetic mechanism in Archaea.