UCARE: Undergraduate Creative Activities & Research Experiences


Date of this Version

Spring 4-4-2020

Document Type



Wulkop Gil CA, Everhart SE (2020). Fungicide Sensitivity of Sclerotinia sclerotiorum Isolates Selected from Five Different States That Use Different Fungicide Treatments. Poster presentation, UCARE Research Fair, Spring 2020, University of Nebraska-Lincoln.


Copyright 2020 by the authors.


Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and different numbers of applications depending on environmental conditions, we hypothesize that isolates with the lowest fungicide sensitivity will be those that come from fields with more intensive fungicide applications. We aim to determine the fungicide sensitivity of S. sclerotiorum isolates from five states to assess risk of resistance. Isolates were selected from dry bean fields from five states from the selection of isolates in the Evertart lab. Isolates were screened against boscalid, tetraconazole, and picoxystrobin fungicides using discriminatory concentrations previously determined by members of the Everhart lab and their EC50(D) was calculated. Interestingly, preliminary results show that the baseline and Washington isolates have a significantly higher EC50(D) than Nebraska isolates when screened against tetraconazole; and that the Washington, Michigan, and Baseline isolates have a significantly higher EC50(D) than Nebraska isolates when screened against picoxystrobin. Differences in EC50(D) in different states hints at S. sclerotiorum developing resistance to fungicides.