UCARE: Undergraduate Creative Activities & Research Experiences

 

Date of this Version

4-2016

Document Type

Poster

Citation

Poster, University of Nebraska-Lincoln Research Fair, Spring 2016.

Comments

Copyright (c) 2016 Samuel Eastman, Guangyong Li, Fan Yang, Josh Herr and James R. Alfano.

Abstract

Clavibacter michiganensis subsp. Nebraskensis (Cmn) is a pathogen responsible for Goss’s Wilt in maize in the high plains. Strains of Clavibacter michiganensis subsp. Nebraskensis that are virulent and non-virulent in maize have been isolated, but the mechanism of this differentiation is not understood. Investigation of the genetic differences between virulent and non-virulent strains is providing an explanation as to how Cmn causes disease. Cmn mutants with reduced or removed virulence have been created using Tn5 transposon transformation to randomly knockout virulence factors. Potential effectors have also been identified in a genomic “virulence island” region by using bioinformatics.

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