U.S. Department of Veterans Affairs
Date of this Version
Journal of Microbiological Methods 81 (2010) 194–196; doi:10.1016/j.mimet.2010.02.005
Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (MФ) phagocytosis of bacteria within a live animal. MФs collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake.