Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi

Authors

Lisa A. Fitzgerald, University of Nebraska-LincolnFollow
Michael V. Graves, University of Massachusetts–LowellFollow
Xiao Li, University of Massachusetts–Lowell
Tamara Feldblyum, The Institute for Genomic Research, Rockville, MD
James Hartigan, Agencourt Bioscience Corporation, Beverly, MA
James L. Van Etten, University of Nebraska-LincolnFollow

Date of this Version

2-20-2007

Comments

Published in Virology 358:2 (February 20, 2007), pp. 459–471. doi:10.1016/j.virol.2006.08.034 Copyright © 2006 Elsevier Inc. Used by permission. http://www.sciencedirect.com/science/journal/00426822

Abstract

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP–glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that approximately 82% of the genes are present in all three viruses.

Supplementary data to accompany this article is archived in this repository as 4 separate documents.