Agronomy and Horticulture, Department of

 

ORCID IDs

https://orcid.org/0000-0002-4090-1765 https://orcid.org/0000-0003-1568-3361

https://orcid.org/0000-0002-0871-0563 https://orcid.org/0000-0003-1392-212X

https://orcid.org/0000-0002-8836-2924 https://orcid.org/0000-0001-7847-1134

https://orcid.org/0000-0002-0875-8495

Document Type

Article

Date of this Version

2021

Citation

Plant Direct. 2021;00:1–12.

Comments

2021 The Authors.

Abstract

Modern plant breeding increasingly relies on genomic information to guide crop im- provement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to in- duce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a her- itable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effective- ness of the mutagenesis system.

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