Agronomy and Horticulture, Department of
ORCID IDs
https://orcid.org/0000-0002-4090-1765 https://orcid.org/0000-0003-1568-3361
https://orcid.org/0000-0002-0871-0563 https://orcid.org/0000-0003-1392-212X
https://orcid.org/0000-0002-8836-2924 https://orcid.org/0000-0001-7847-1134
https://orcid.org/0000-0002-0875-8495
Document Type
Article
Date of this Version
2021
Citation
Plant Direct. 2021;00:1–12.
Abstract
Modern plant breeding increasingly relies on genomic information to guide crop im- provement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to in- duce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a her- itable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effective- ness of the mutagenesis system.
Included in
Agricultural Science Commons, Agriculture Commons, Agronomy and Crop Sciences Commons, Botany Commons, Horticulture Commons, Other Plant Sciences Commons, Plant Biology Commons
Comments
2021 The Authors.