Agronomy and Horticulture, Department of

 

Document Type

Article

Date of this Version

6-5-2017

Citation

Vol 7, Iss 11, Jun 05, 2017 DOI:10.21769/BioProtoc.2315

Abstract

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

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