Evaluation of genotype quality parameters for SowPro90, a new genotyping array for swine

Authors

Hiruni R. Wijesena, University of Nebraska - LincolnFollow
Gary A. Rohrer, USDA, Agricultural Research Service
Dan J. Nonneman, USDA, Agricultural Research Service
Brittney N. Keel, USDA, Agricultural Research Service
Jessica L. Petersen, University of Nebraska - LincolnFollow
Stephen D. Kachman, University of Nebraska-LincolnFollow
Daniel C. Ciobanu, University of Nebraska-LincolnFollow

ORCID IDs

Jessica L. Petersen

Daniel C. Ciobanu

Date of this Version

2019

Citation

J. Anim. Sci. 2019.97:3262–3273

Comments

© The Author(s) 2019.

This document is a U.S. government work and is not subject to copyright in the United States.

doi: 10.1093/jas/skz185

Abstract

Understanding early predictors of sow fertility has the potential to improve genomic predictions. A custom SNP array (SowPro90 produced by Affymetrix) was developed to include genetic variants overlapping quantitative trait loci for age at puberty, one of the earliest indicators of sow fertility, as well as variants related to innate and adaptive immunity. The polymorphisms included in the custom genotyping array were identified using multiple genomic approaches including deep genomic and transcriptomic sequencing and genome-wide associations. Animals from research and commercial populations (n = 2,586) were genotyped for 103,476 SNPs included in SowPro90. To assess the quality of data generated, genotype concordance was evaluated between the SowPro90 and Porcine SNP60 BeadArray using a subset of common SNP (n = 44,708) and animals (n = 277). The mean genotype concordance rate per SNP was 98.4%. Differences in distribution of data quality were observed between the platforms indicating the need for platform specific thresholds for quality parameters. The optimal thresholds for SowPro90 (≥97% SNP and ≥93% sample call rate) were obtained by analyzing the data quality distribution and genotype concordance per SNP across platforms. At ≥97% SNP call rate, there were 42,151 SNPs (94.3%) retained with a mean genotype concordance of 98.6% across platforms. Similarly, ≥94% SNPs and ≥85% sample call rates were established as thresholds for Porcine SNP60 BeadArray. At ≥94% SNPs call rate, there were 41,043 SNPs (91.8%) retained with a mean genotype concordance of 98.6% across platforms. Final evaluation of SowPro90 array content (n = 103,476) at ≥97% SNPs and ≥93% sample call rates allowed retention of 89,040 SNPs (86%) for downstream analysis. The findings and strategy for quality control could be helpful in identifying consistent, high-quality genotypes for genomic evaluations, especially when integrating genotype data from different platforms.