Thirty-Seven Additional Microsatellite Loci in the Pacific Lion-Paw Scallop (Nodipecten subnodosus) and Cross-Amplification in Other Pectinids

Authors

Jessica Lynn Petersen, University of Nebraska-LincolnFollow
Ana M. Ibarra, Centro de Investigaciones Biológicas del Noroeste S.C
Bernie May, University of California - Davis

Date of this Version

2009

Citation

Conservation Genetics Resources (2009) 1: 101-105. DOI: 10.1007/s12686-009-9025-8.

Comments

Copyright 2009, the authors. Used by permission. Technical note.

Abstract

We characterized 37 new microsatellite markers in the Pacific lion-paw scallop (Nodipecten subnodosus) and tested for cross-amplification in four other species. Genetic diversity was estimated using 24 individuals from the Lagoon Ojo de Liebre, B.C.S., Mexico. Allelic richness varied from 5 to 27 alleles per locus and the average expected heterozygosity was 0.76. Ten loci exhibited significant departure from Hardy–Weinberg equilibrium likely due to the presence of null alleles. Sixteen of these markers cross-amplified in closely related N. nodosus, while little or no amplification was observed in three Argopecten species.