Characterization of 35 Microsatellite Loci in the Pacific Lion-Paw Scallop (Nodipecten subnodosus) and Their Cross-Species Amplification in Four Other Scallops of the Pectinidae Family

Authors

Ana M. Ibarra, Centro de Investigaciones Biológicas del Noroeste S.C.Follow
Jessica Lynn Petersen, University of Nebraska-LincolnFollow
Thomas R. Famula, University of California - Davis
Bernie May, University of California - Davis

Date of this Version

2006

Citation

Molecular Ecology Notes (2006) 6: 153-156. DOI: 10.1111/j.1471-8286.2005.01173.x.

Comments

Copyright 2006, Wiley. Used by permission.

Abstract

Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy–Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyze the population structure of the species in Mexico’s Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.