Characterization of 24 Microsatellite Loci in Delta Smelt, Hypomesus transpacificus, and Their Cross-Species Amplification in Two Other Smelt Species of the Osmeridae Family

Authors

Kathleen M. Fisch, Scripps Research InstituteFollow
Jessica Lynn Petersen, University of Nebraska-LincolnFollow
Melinda R. Baerwald, University of California - Davis
John K. Pedroia, University of California - Davis
Bernie May, University of California - Davis

Document Type

Article

Date of this Version

2009

Citation

Molecular Ecology Resources (2009) 9: 405-408. DOI: 10.1111/j.1755-0998.2008.02254.x.

Comments

Copyright 2009, Wiley. Used by permission.

Abstract

We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, California, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).