Animal Science Department

 

Date of this Version

2002

Citation

J. Anim. Sci. 2002. 80:1379–1380.

Comments

Copyright 2002 American Society of Animal Science. All rights reserved. Used by permission.

Abstract

Locus. Pig Tropomyosin beta (TPM2) gene.

Genus and Species. Sus Scrofa.

Source and Description of Primers. Primers were derived by using well-conserved regions of published human and mouse sequence (GenBank accession nos. AF209746 and NM_009416). The primers were used to amplify a 1.4-kp fragment of the porcine TPM2 gene covering exons 3 through 8. The porcine cDNA sequence (GenBank accession no. AF420022) showed 95% exonic identity (325 bp) at the nucleotide level to the human skeletal muscle beta tropomyosin (GenBank accession no. X06825). Additionally, pig specific primers were designed to amplify a 246-bp piece that spans exons 3 to 4 (GenBank accession # AF420023) to be used for linkage and physical mapping.

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