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Four experiments were conducted to evaluate effects of in situ bag rinsing technique and sample size on the variation of undegradable intake protein (UIP) and dry matter disappearance (DMD) of soybean meal (SBM) and Soypass, a heat-treated soybean meal. Five rinsing techniques and five sample sizes were used to test effects. Soybean meal had higher DMD, lower UIP and higher variance for UIP than Soypass. A steer difference was noted for experiments with steer as a replication and also contributed a larger effect than day and run within day. Rinsing technique and sample size were not significant in concentrate fed steers but were in mixed diet steers. There was a rinsing difference with highest machine rinses having higher DMD and lower UIP values. A size difference was noted with largest sample size having lowest DMD and highest UIP. No difference was found between hand and machine rinsing and no evidence was found to eliminate the use of an increased sample size.