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The objectives of this study were to determine if post mortem temperature affects extent of glycogen metabolism and if sampling time influences glycolytic potential values in muscle. Beef longissimus muscles entered rigor mortis at two different temperatures and were sampled at 45 minutes post mortem, rigor mortis and 24 hours post mortem to determine the glycolytic potential of the muscle. Post mortem temperature had little effect on the glycolytic potential of beef muscle. Glycolytic potential values from samples removed early post mortem were underestimated when compared to samples taken at 24 hours post mortem.