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Tympanic and vaginal temperature logging devices were used to collect internal body temperature in three trials using mature nonpregnant beef cows. A model was developed to predict daily patterns for internal body temperature of a cow as a function of ambient temperature. Panting scores were recorded and differed across days as cows experienced changes in ambient temperature and humidity. Vaginal and tympanic temperatures were positively correlated, thus tympanic temperature may be used to predict internal body temperature of cows.